What are the Limitations of the Hershberger Assay?
Despite its usefulness, the Hershberger Assay has some limitations:
In vivo Nature: As an in vivo assay, it involves animal use, which raises ethical concerns. Specificity: While it provides information on androgenic activity, it does not offer insights into other hormonal activities such as estrogenic or thyroid-disrupting effects. Time and Cost: The assay is time-consuming and resource-intensive compared to in vitro methods.
Researchers must weigh these limitations against the assay's benefits when designing toxicological studies.
