Cell Culture - Toxicology

What is Cell Culture?

Cell culture involves the growing of cells under controlled conditions, usually outside their natural environment. In the context of toxicology, cell culture is a pivotal technique used to study the effects of toxic substances on cells.

Why is Cell Culture Important in Toxicology?

Cell culture provides a controlled environment to study the interaction of chemicals with biological systems. It helps in understanding the mechanisms of toxicity, allows for the testing of a wide range of substances, and reduces the need for animal testing.

Types of Cells Used

Different types of cells are used depending on the nature of the study. These include primary cells, which are directly taken from organisms, and immortalized cell lines, which can proliferate indefinitely. Each type has its own advantages and limitations.

Applications of Cell Culture in Toxicology

One major application is in the screening of potential drug candidates for toxicity. Cell culture is also used to study the effects of environmental toxins and to understand the cellular response mechanisms to toxicants.

Challenges and Limitations

While cell culture is a powerful tool, it has limitations. For example, lacking the complexity of a whole organism can sometimes result in misleading data. Additionally, maintaining cell cultures can be costly and labor-intensive.

Future Directions

Advancements in 3D cell culture technologies and organoids are promising to overcome some of the current limitations. These technologies aim to create more complex and representative models of human tissues, thereby providing more reliable data on the effects of toxic substances.

Conclusion

Cell culture continues to be an invaluable tool in toxicology research. Despite its challenges, it offers a controlled, ethical, and versatile method for studying the effects of toxic substances on biological systems, helping to advance our understanding and development of safer drugs and chemicals.

Relevant Publications

Retinal Explant Culture from Mouse, Human, and Nonhuman Primates and Its Applications in Vision Research.

Issue Release: 2025

Temperature-responsive hydrogel-grafted vessel-on-a-chip: Exploring cold-induced endothelial injury.

Issue Release: 2024

Human gut Actinobacteria boost drug absorption by secreting P-glycoprotein ATPase inhibitors.

Issue Release: 2024

A rare presentation of isolated candidal hepatic abscess in a maintenance hemodialysis patient.

Issue Release: 2024

Ectoine enhances recombinant antibody production in Chinese hamster ovary cells by promoting cell cycle arrest.

Issue Release: 2024

Cytokinins enhance the metabolic activity of in vitro-grown catmint (Nepeta nuda L.).

Issue Release: 2024

Distinct roles and molecular mechanisms of nicotine and benzo(a)pyrene in ferroptosis of lung adenocarcinoma and lung squamous cell carcinoma.

Issue Release: 2024

Establishment of a humanized mouse model using steady-state peripheral blood-derived hematopoietic stem and progenitor cells facilitates screening of cancer-targeted T-cell repertoires.

Issue Release: 2024

Fluid-Dynamic Modeling of Flow in Embryonic Tissue Indicates That Lymphatic Valve Location Is Not Consistently Determined by the Local Fluid Shear or Its Gradient.

Issue Release: 2024

Identification and validation of tumor-specific T cell receptors from tumor infiltrating lymphocytes using tumor organoid co-cultures.

Issue Release: 2024

Utilizing human cerebral organoids to model breast cancer brain metastasis in culture.

Issue Release: 2024

Immunomodulatory effects of antiangiogenic tyrosine kinase inhibitors in renal cell carcinoma models: Impact on following anti-PD-1 treatments.

Issue Release: 2024

Pitavastatin sensitizes the EGFR-TKI associated resistance in lung cancer by inhibiting YAP/AKT/BAD-BCL-2 pathway.

Issue Release: 2024

Computational model of the cancer necrotic core formation in a tumor-on-a-chip device.

Issue Release: 2024

Targeting tumor-associated macrophage with mannosylated nanotherapeutics delivering TLR7/8 agonist enhances cancer immunotherapy.

Issue Release: 2024

\"Glia-like\" cells of fibroblast morphology are present in cultures from injured human brain tissue.

Issue Release: 2024

Inhibition of BACE1 affected both its Aβ producing and degrading activities and increased Aβ42 and Aβ40 levels at high level BACE1 expression.

Issue Release: 2024

The Phenol Red Compound: A Potential Artifact in Pharmacological Induction of Ferroptosis.

Issue Release: 2024

BACH1 inhibits senescence, obesity, and short lifespan by ferroptotic FGF21 secretion.

Issue Release: 2024

Umbilical cord mesenchymal stem cell exosomes alleviate necrotizing enterocolitis in neonatal mice by regulating intestinal epithelial cells autophagy.

Issue Release: 2024

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