The procedure for conducting a biochemical assay typically involves several steps:
Sample Preparation: Biological samples, such as blood, urine, or tissue, are collected and prepared for analysis. Reagent Addition: Specific reagents are added to the sample to react with the target molecule. Incubation: The sample is incubated for a specific period to allow the reaction to occur. Detection: The reaction product is detected using various methods, such as spectrophotometry, fluorometry, or chemiluminescence. Data Analysis: The results are analyzed to determine the concentration or activity of the target molecule.
