How are SH-SY5Y Cells Cultured and Differentiated?
Culturing SH-SY5Y cells involves growing them in standard culture mediums like DMEM/F12 supplemented with fetal bovine serum (FBS). To induce differentiation, researchers often expose the cells to agents such as retinoic acid or brain-derived neurotrophic factor (BDNF). Differentiation enhances the neuronal characteristics of these cells, making them more suitable for neurotoxicity studies.
