There are several types of 3D culture systems, including scaffold-based, scaffold-free, and organ-on-a-chip platforms. Scaffold-based systems use biomaterials to support cell growth in three dimensions, while scaffold-free systems rely on cell-cell interactions to form 3D structures. Organ-on-a-chip technology integrates microfluidics to simulate the physiological conditions of human organs. These systems can be used to culture a variety of cell types, including cancer cells, stem cells, and primary cells, making them versatile tools in toxicology research.
