Chromatography operates on the principle of partitioning components between a mobile phase and a stationary phase. In toxicology:
- Sample Preparation: Biological samples are prepared and possibly pre-treated to remove proteins and other interfering substances. - Injection: The prepared sample is injected into the chromatographic system. - Separation: The sample components travel through the stationary phase at different rates, leading to separation. - Detection: Detectors such as UV-Vis, fluorescence, or mass spectrometry are used to identify and quantify the components.
