single cell rna sequencing

How does scRNA-seq Work?


scRNA-seq involves isolating individual cells from a tissue or culture, capturing their mRNA, and then sequencing it to determine the gene expression profile of each cell. This process typically includes cell dissociation, mRNA capture, reverse transcription, amplification, and sequencing. Advances in technology have streamlined these steps, allowing for high-throughput sequencing of thousands of cells simultaneously.

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