How is Cell Membrane Integrity Assessed in Toxicology?
Several assays and techniques are employed to assess cell membrane integrity:
Dye Exclusion Tests: Assays such as Trypan Blue exclusion or Propidium Iodide staining are used to determine cell viability based on membrane integrity. Lactate Dehydrogenase (LDH) Release Assay: The release of LDH into the extracellular medium is an indicator of membrane damage. Fluorescence Microscopy: Fluorescent dyes can be used to visualize membrane integrity and permeability changes in cells.
