The process of histopathological analysis involves several steps: 1. Sample Collection: Tissue samples are collected from subjects exposed to the toxic agent. 2. Fixation: The tissue is preserved using fixatives to prevent degradation. 3. Embedding: The fixed tissue is embedded in a medium, usually paraffin, to provide support during sectioning. 4. Sectioning: Thin slices of the tissue are cut using a microtome. 5. Staining: Sections are stained using dyes to highlight different cellular components. 6. Microscopic Examination: The stained sections are examined under a microscope to identify pathological changes.
