hen's egg test on the chorioallantoic membrane (het cam)

How is the HET-CAM Test Conducted?

The procedure begins with incubating fertilized hen's eggs for around nine days until the CAM is fully developed. The eggshell is carefully opened to expose the CAM, and the test substance is applied. Observations are made for up to five minutes, noting any irritation signs such as hemorrhage, coagulation, or lysis of blood vessels. The responses are scored, and the degree of irritation is categorized based on established scales.

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