Ethical Considerations: HET-CAM reduces the need for live animal testing, addressing ethical concerns related to animal welfare. Cost-Effectiveness: The test is less expensive than traditional in vivo methods, making it accessible for routine screening. Rapid Results: HET-CAM provides quick results, often within hours, facilitating timely decision-making in product development. Predictive Value: The test has shown good correlation with ocular irritation potential in humans, enhancing its applicability in safety assessments.