Specificity: Probes must be highly specific to their target to avoid false positives. This is achieved by designing molecular structures that bind selectively to the target molecule. Sensitivity: The probe should be sensitive enough to detect low concentrations of the target molecule, which is crucial in detecting early signs of toxicity. Cell Permeability: Effective probes must penetrate cell membranes without altering cell viability or function. Photostability: Probes should maintain their fluorescent properties under prolonged exposure to light to allow for extended observation periods.
