What are the Limitations of Masson's Trichrome Staining?
While Masson's Trichrome staining is a valuable tool, it has its limitations:
Quantitative Limitations: The technique is mainly qualitative. Quantifying the extent of fibrosis requires additional image analysis techniques. Specificity Concerns: Although effective in highlighting collagen, the stain may not differentiate between various types of collagen fibers. Time-Consuming Process: The staining process can be time-consuming and requires precise conditions to ensure reproducibility.
