What Are the Limitations of FISH in Toxicological Studies?
Despite its advantages, FISH also has some limitations. The technique requires prior knowledge of the DNA sequence of interest to design specific probes, which can be a constraint when exploring unknown genetic regions. FISH is also limited by its resolution; while it can identify large chromosomal changes, it may not detect smaller mutations or single nucleotide polymorphisms. Additionally, the preparation and analysis of samples can be labor-intensive and time-consuming, which may be a drawback for high-throughput screening.
