What Challenges are Associated with Multi-Color Imaging?
Despite its advantages, multi-color imaging poses several challenges. One major issue is spectral overlap, where the emission spectra of different dyes overlap, complicating the separation of signals. To address this, researchers must carefully select fluorescent labels with distinct spectra and employ advanced microscopy techniques. Moreover, the introduction of multiple dyes can potentially interfere with cellular processes, necessitating rigorous validation of experimental conditions.
