Ecotoxicologists face several challenges, including the complexity of ecosystems, the vast number of potential chemicals, and the variability in sensitivity among species. The complexity of ecosystems makes it difficult to isolate the effects of a single chemical, as multiple factors can influence outcomes. Additionally, with thousands of chemicals in use, prioritizing which ones to study can be daunting. Differences in sensitivity among species mean that results from one organism cannot always be generalized to others, necessitating a wide range of studies across different species.