What Staining Techniques are Used in Toxicological Light Microscopy?
Several staining techniques are routinely used, including Hematoxylin and Eosin (H&E) staining, which provides an overview of tissue architecture and cellular morphology. Special stains like Periodic Acid-Schiff (PAS), Masson's Trichrome, and Prussian Blue are used to highlight specific tissue components such as carbohydrates, collagen, and iron deposits, respectively. These stains help in identifying specific toxic-induced changes in tissues.