Despite its advantages, Oil Red O staining has some limitations. It is primarily qualitative, providing visual evidence of lipid presence without quantifying the amount. Additionally, it may not differentiate between types of lipids, such as phospholipids and neutral lipids. Furthermore, the fixation and processing steps can sometimes lead to lipid loss, potentially affecting the accuracy of the results. Despite these challenges, the method remains a standard approach in many toxicological assessments.
