The process of Oil Red O staining involves several steps. Tissue samples are first fixed in a formaldehyde-based solution, followed by cryosectioning. The sections are then stained with an Oil Red O solution, which specifically binds to and colors lipids. After staining, the sections are rinsed and counterstained to enhance contrast. This technique allows researchers to visualize lipid droplets within tissues, providing insight into the pathological changes associated with toxin exposure.
