Are There Limitations to Using Fluorescent Proteins?
While fluorescent protein technology has many benefits, it also has limitations:
Photobleaching: Prolonged exposure to light can cause the fluorescent signal to fade, potentially compromising data quality. Phototoxicity: Intense light exposure can damage cells, affecting the interpretation of results. Protein Function Alteration: Tagging proteins with fluorescent markers may alter their function or localization, potentially skewing experimental results.
