Subacute and subchronic toxicity tests involve specific methodologies:
Animal Models: Commonly, rodents like rats and mice are used due to their biological similarities to humans and practicality in laboratory settings. Exposure Routes: The route of exposure (oral, dermal, inhalation) is chosen based on how humans are likely to encounter the substance. Parameters Measured: Observations may include changes in body weight, food consumption, hematological and biochemical parameters, organ weights, and histopathological examination of tissues. Data Analysis: Results are analyzed to determine dose-response relationships and identify potential target organs or systems.
