Toxicological risk assessment is a crucial process for evaluating the safety of man-made derivatives. This involves determining the dose-response relationship, which helps to identify the concentration at which a substance becomes toxic. Additionally, assessments consider factors such as exposure duration, frequency, and routes (e.g., inhalation, ingestion, dermal contact). Regulatory agencies, including the Environmental Protection Agency (EPA) and the Food and Drug Administration (FDA), use these assessments to establish safety guidelines and permissible exposure limits.