Toxicity studies involve various methodologies, including in vitro (test tube or cell culture) and in vivo (animal) models. The choice of model depends on the type of study and the specific endpoints being evaluated. Key steps in conducting toxicity studies include:
1. Selection of Test Substance: Identifying the substance to be tested, including its formulation and purity. 2. Dose Selection: Determining appropriate doses for testing, often based on prior pharmacokinetic and toxicity data. 3. Exposure Route: Deciding the method of exposure, such as oral, inhalation, or dermal. 4. Observation and Data Collection: Monitoring test subjects for adverse effects and collecting data on various parameters such as body weight, clinical signs, and histopathology. 5. Data Analysis: Analyzing the collected data to identify toxic effects and establish dose-response relationships.
