BSCs function by using high-efficiency particulate air (HEPA) filters to capture and contain airborne particles, including microorganisms and chemical vapors. There are three main types of BSCs, each designed for different levels of protection and types of work:
1. Class I BSCs: These provide personnel and environmental protection but do not offer product protection. They are suitable for work with low to moderate-risk biological materials. 2. Class II BSCs: These are the most commonly used BSCs in toxicology. They offer protection for personnel, environment, and product. Class II BSCs are suitable for handling hazardous microorganisms and low-level chemical compounds. 3. Class III BSCs: Also known as glove boxes, these provide the highest level of protection and are used for handling highly infectious or toxic materials. They are completely sealed and require operators to use long gloves attached to the cabinet.