How Does Autofluorescence Affect Toxicological Studies?
While autofluorescence can provide useful information, it can also interfere with fluorescence-based assays. For instance, when assessing the effects of toxins using fluorescent dyes, the natural autofluorescence of tissues can mask or mimic the signals from the dyes, leading to misleading results. Therefore, it is crucial to distinguish between autofluorescent signals and those from specific markers.