What Techniques are Used to Manage Autofluorescence?
Several strategies are employed to manage autofluorescence in toxicological studies:
Spectral Unmixing: This technique involves separating the autofluorescent signals from those of the fluorescent dyes based on their distinct emission spectra. Photobleaching: This method reduces autofluorescence by exposing the sample to light until the autofluorescent components are bleached, although care must be taken not to damage other fluorescent signals. Advanced Imaging Techniques: Techniques such as confocal microscopy and two-photon microscopy can enhance specificity and resolution, helping to differentiate autofluorescent signals.
