Compared to other immunoassays, such as enzyme-linked immunosorbent assay (ELISA), IFA provides better spatial resolution, allowing for the visualization of antigen localization within cells or tissues. However, ELISA is often preferred for quantitative analysis due to its simplicity and ability to be automated. Mass spectrometry offers higher sensitivity and specificity for small molecule detection but lacks the ability to provide spatial information. Thus, the choice of technique depends on the specific requirements of the study.
