The process of conducting a bioassay involves several key steps:
Selection of Test Organism: The choice of organism depends on the substance being tested and the specific endpoints of interest, such as mortality, growth inhibition, or behavioral changes. Exposure to Substance: The test organism is exposed to varying concentrations of the substance, often using a control for comparison. Observation and Data Collection: Researchers observe the organism for specific responses and collect data on parameters such as survival rate, growth rate, and physiological changes. Data Analysis: Statistical methods are used to analyze the data and determine dose-response relationships, often resulting in the calculation of the median lethal dose (LD50) or other relevant metrics.
