Assessment of biological relevance involves several steps:
Dose-response relationship: Analyzing the relationship between the dose of a substance and the observed effect. Mechanism of action: Understanding how a substance causes an effect at the molecular, cellular, or organismal level. Comparative biology: Comparing the biological systems of test organisms (e.g., rodents) with humans to evaluate relevance. Exposure assessment: Evaluating the levels and routes of human exposure to determine real-world applicability.
