1. Sample Collection: Depending on the type of test, a sample of urine, blood, hair, saliva, or sweat is collected. 2. Sample Preparation: The sample is then prepared for analysis, which may involve processes such as dilution, extraction, or purification. 3. Detection and Analysis: Advanced analytical techniques like Gas Chromatography (GC), Mass Spectrometry (MS), or Immunoassays are employed to detect and quantify the presence of specific drugs or their metabolites. 4. Interpretation of Results: The results are interpreted by toxicologists to determine the presence and concentration of drugs, and appropriate actions are taken based on these findings.