The process of H&E staining involves several steps. First, tissues are fixed, typically in formaldehyde, to preserve their structure. They are then embedded in paraffin, sectioned into thin slices, and mounted on slides. The slides undergo a series of staining procedures, starting with hematoxylin staining, which highlights cell nuclei. After rinsing, eosin is applied to stain other tissue components. The slides are then dehydrated, cleared, and mounted for microscopic examination.
