In the field of toxicology, histopathological analysis is crucial for understanding the impact of toxic substances on biological tissues. One of the most widely used staining techniques is hematoxylin and eosin (H&E) staining, which allows for the detailed visualization of tissue architecture. Hematoxylin stains cell nuclei blue, while eosin stains the cytoplasm and extracellular matrix pink, providing a clear contrast that facilitates the examination of tissue morphology.