The IHC process involves several steps: tissue fixation, sectioning, blocking, antibody application, and detection. First, tissues are fixed to preserve cellular structure. Sections are then cut and treated with a blocking agent to prevent non-specific binding. Primary antibodies are applied to target antigens, followed by secondary antibodies linked to enzymes or dyes for detection. Finally, substrates are added to produce a visible signal where the antibody-antigen complexes form.
