Several techniques are used to measure plasma protein binding, including: - Equilibrium dialysis: This method measures the free and bound fractions by allowing the drug to reach equilibrium between plasma and a protein-free buffer. - Ultrafiltration: This technique separates free drug from bound drug using a filter that allows only small molecules to pass. - Ultracentrifugation: High-speed centrifugation separates bound and unbound fractions based on their size and density differences.
