Subacute lethality is typically measured through animal studies, where subjects are exposed to a substance for a period ranging from 14 to 28 days. Key parameters include:
1. Dose-Response Relationship: Determining the relationship between the dose and the severity of toxic effects. 2. LD50 (Lethal Dose 50%): The dose at which 50% of the test population succumbs to the substance. Though more commonly associated with acute toxicity, it can also be relevant in subacute studies. 3. Clinical Observations: Monitoring symptoms such as weight loss, behavioral changes, and organ malfunction. 4. Histopathological Analysis: Examining tissues and organs for microscopic changes indicative of toxicity.
