Selecting an appropriate positive control depends on several factors:
1. Nature of the Test: The positive control should be relevant to the biological mechanism being studied. For example, if the study is focused on oxidative stress, a substance known to induce oxidative stress should be chosen.
2. Concentration: The concentration of the positive control should be within a range that produces a measurable effect without causing excessive damage or systemic toxicity that could confound the results.
3. Stability and Availability: The positive control should be stable under the experimental conditions and readily available for consistency across different experiments.
