What are Alternative Methods to Measure Lipid Peroxidation?
Given the limitations of the TBARS assay, alternative methods are often used to measure lipid peroxidation. These include high-performance liquid chromatography (HPLC) to directly measure malondialdehyde, and gas chromatography-mass spectrometry (GC-MS) for more specific detection of lipid peroxidation products. Other biomarkers like F2-isoprostanes and 4-hydroxynonenal (4-HNE) are also used to provide a more comprehensive assessment of lipid oxidative damage.
