Fluorophores are molecules that can re-emit light upon light excitation. They have distinct excitation and emission spectra, which makes them highly useful in various scientific fields, including toxicology. Fluorophores can be naturally occurring, like the amino acid tryptophan, or synthetic, such as fluorescein and rhodamine. These compounds are designed to bind to specific molecules or structures, allowing researchers to track and quantify them with high sensitivity and specificity.