Sample preparation in toxicology can be challenging due to several factors: 1. Complexity of Biological Matrices: Biological samples such as blood, urine, and tissues are complex and contain various substances that can interfere with the analysis. 2. Trace Levels of Analytes: Often, the analytes of interest are present in trace amounts, requiring highly sensitive and selective preparation methods. 3. Degradation of Analytes: Some analytes can degrade quickly, necessitating rapid and efficient preparation techniques to preserve their integrity. 4. Variability: Biological samples can vary significantly between individuals, adding another layer of complexity to the preparation process.
