What are the Common Methods of Sample Preparation?
Several methods are used in sample preparation, depending on the type of sample and the analyte of interest:
1. Liquid-Liquid Extraction (LLE): This method involves the separation of compounds based on their solubility in two different immiscible liquids. 2. Solid-Phase Extraction (SPE): This technique uses solid adsorbents to separate analytes from a liquid mixture, providing high selectivity and sensitivity. 3. Protein Precipitation: Commonly used for the removal of proteins from biological samples, which can interfere with the analysis. 4. Derivatization: Involves chemically modifying analytes to make them more detectable during analysis. 5. Filtration and Centrifugation: Removes particulates and other impurities from samples to avoid clogging and contamination of analytical instruments.
