One of the main challenges in studying disruption in toxicology is the complexity of biological systems and the variability in responses to toxicants. Different species and even individuals within a species can have varying sensitivities to the same toxicant. Additionally, the effects of disruption may not be immediately apparent and can manifest over long periods. This complexity necessitates sophisticated experimental designs and analytical methods to accurately assess the impact of toxicants. Another challenge is the regulation and management of chemicals, which require comprehensive risk assessments to determine safe exposure levels.