Despite their importance, conducting toxicological assays can be challenging:
Complexity of biological systems: Living organisms are complex, and it can be difficult to predict how they will respond to a substance based solely on laboratory assays. Ethical considerations: The use of animals in testing raises ethical concerns, prompting the development of alternative methods like in vitro and in silico assays. Interpreting data: Toxicological data can be complex and require expertise to interpret, especially when results from different assays conflict.