What Are the Challenges in Using Pathway-Focused Assays?
While pathway-focused assays offer valuable insights, they also present challenges. One significant challenge is the complexity of biological systems, which can make it difficult to isolate the effects of a single pathway. Another challenge is the interpretation of data, as changes in pathway activity may not always correlate directly with adverse outcomes. Additionally, the integration of data from multiple assays to provide a comprehensive toxicological profile requires sophisticated analytical tools and expertise.