What are the Key Steps in Performing a TUNEL Assay?
The TUNEL assay involves several key steps:
Sample Preparation: Cells or tissue samples are fixed to preserve cellular structures. Permeabilization: The cell membrane is permeabilized to allow access to the DNA. Labeling: Terminal deoxynucleotidyl transferase (TdT) enzyme adds labeled dUTP to the ends of fragmented DNA. Detection: The labeled DNA fragments are detected using fluorescence microscopy or flow cytometry.
