fluorescence imaging

What Are the Limitations of Fluorescence Imaging?


Despite its benefits, fluorescence imaging has certain limitations. One significant challenge is the photobleaching of fluorescent dyes, where prolonged exposure to light causes the dyes to lose their fluorescence intensity. Additionally, autofluorescence from biological samples can interfere with signal detection, complicating the interpretation of results. The resolution of fluorescence imaging is also limited by the diffraction of light, although advances in super-resolution techniques are addressing this issue.

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