What Are the Limitations of Staining Techniques in Toxicology?
Despite their utility, staining methods have certain limitations:
Lack of Specificity: Some stains might lack specificity, making it challenging to distinguish between different cell types or structures. Technical Variability: Staining results can vary based on technique, reagent quality, and the experience of the technician, potentially affecting reproducibility. Quantitative Analysis: While staining provides qualitative data, quantitative analysis may require additional methods, such as image analysis software or biochemical assays.