The biopanning process typically involves the following steps:
Immobilization of the target molecule, such as a toxin or receptor, on a solid support. Incubation of the phage display library with the immobilized target to allow binding of specific peptides. Washing to remove unbound and weakly bound phages, ensuring that only those with high affinity are retained. Elution of the bound phages, which are then amplified in bacterial hosts. Subsequent rounds of panning may be performed to enhance the specificity and affinity of the selected peptides.
