In Vivo Assays: These involve testing the effects of substances on living organisms, such as rodents or fish. They provide comprehensive data on the systemic effects of toxins. In Vitro Assays: Conducted in a controlled laboratory environment, these assays use cells or tissues to evaluate the toxic potential of substances, offering a quicker and more ethical alternative to in vivo testing. In Silico Assays: Utilizing computer models, these assays predict the toxicological effects based on existing data, helping to reduce the need for animal testing.