What Challenges are Associated with H&E Staining in Toxicology?
Despite its widespread use, H&E staining has limitations and challenges. One major challenge is the subjective nature of interpretation, which relies heavily on the experience of the pathologist. Additionally, while H&E provides excellent morphological detail, it lacks the specificity to identify particular biomolecules or cell types, which may require complementary techniques such as immunohistochemistry or in situ hybridization.
