Acute toxicity testing is a fundamental aspect of toxicology that involves evaluating the adverse effects of a substance following a short-term exposure. Typically, this testing assesses the immediate or short-term health risks of a substance after a single dose or multiple doses within 24 hours. The primary objective is to determine the lethal dose (LD50), which is the dose required to kill 50% of the test population, and to understand the symptoms and potential effects of acute exposure.